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immersion

In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.
Without oil, light waves reflect off the slide specimen through the glass cover slip, through the air, and into the microscope lens (see the colored figure to the right). Unless a wave comes out at a 90-degree angle, it bends when it hits a new substance, the amount of bend depending on the angle. This distorts the image. Air has a very different index of refraction from glass, making for a larger bend compared to oil, which has an index more similar to glass. Specially manufactured oil can have nearly exactly the same refractive index as glass, making an oil immersed lens nearly as effective as having entirely glass to the sample (which would be impractical).
Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy. Typical oils used have an index of refraction of around 1.515. An oil immersion objective is an objective lens specially designed to be used in this way. Many condensers also give optimal resolution when the condenser lens is immersed in oil.

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  1. M

    immersion heater thermostat keeps tripping on..

    A direct unvented. I am thinking that the element is just full of scale so keeps over heating and cutting out. But is the way to test these by resistance on coil and resistance across thermostat? I have read on here that testing on points with casing is good? What does this do? Is the casing...
  2. M

    Urgent help needed please

    Evening guys, got a bit of a problem ive come home tonight to find drops of water coning through the room ceiling the house is a bungolow, i went into the attic to find a tank full to the brim and slowly coming over the side and onto the floorboards ! Now I dont have a clue about plumbing...
  3. S

    Immersion Heater sounds like a kettle

    Afternoon all, and thanks in advance for your help. About 2 years ago, the immersion heater in our hot water cylinder broke so we had it replaced. Now I can't remember 100% but I'm sure that before the change, when we put the immersion on it didn't make much noise. Since the change, as soon...
  4. D

    Range Tribune Immersion Element Thread Adaptor

    Don't suppose anyone out there has come across this infuriating company who uses non-standard sized immersion elements in their unvented cylinders? The thread is (I think!) 1 3/4" BSP where a standard one is 2 1/4". Given that I can get hold of replacement elements from PTS but they are £50...
    • Drip Doctor
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    • adaptor element elements immersion range standard these thread
    • Replies: 13
    • Forum: Plumbing Jobs | The Job-board
  5. P

    hot water problems

    Hi, I figured this would be the best place to try get this sorted, hope someone can help. We put the heating on and everything works fine, water gets hot, radiators get hot etc...If we turn it off then everything gets cold.*The problem is that over the last few days we have noticed that it gets...
  6. D

    Please help. Hot water in bath only fills up one quarter of tub then goes off

    Hello all. Please if anyone can help... I moved into a social housing flat with my little boy last June, and we don't have a shower, only a bath tub, and the hot water always runs out at about a quarter full (the flow literally stops) and all you get is cold water from the other tap. I have...
  7. S

    Immersion Heater problem.

    I have just connected up a large immersion heater. A plumber installed this on a house refurb. ( who the owner can't get hold of right now) The immersion is controlled by a horstmann eco7 with a boost (top element) and economy 7 timer(bottom element) At first the home owners couldn't get any...
  8. R

    Immersion issue

    Hi Friends So, after having no drain tee on a combi tank I put the hosepipe down the cold internal feed and sucked, hey presto after a couple of goes it started to drain, all 100%. Now, after fitting the new immersion and thermostat (that is already fitted) and wiring the other bits up I...
  9. H

    Immersion

    Fitted a lower complete immersion the end of last month for a tenant.....all good. Tenant rang me last week saying it wasn't working, so I went around and on closer inspection there is very slight weeps from the live/neutral connections of the element. Told the tenant I will sort it. Went to...
  10. M

    Elson Zircon immersion element

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  11. S

    immersion

    on a 48 x 18 direct cylinder will you fit a 27" or 36" immersion heater ?
  12. I

    immersion cylinder

    Bit of a problem. fitted a new immersion tank and after a bit of a struggle and one or two drips ( sorted now ), I am not getting any hot water, flow pipe on the coil hot but return cold, im thinking an air lock but there is no vent, how best to get rid of airlock. Advice greatly appreciated
  13. C

    immersion heater testing with multimeter

    hi all , sent to a job today to change 2 immersion elements in a 210 tank , went there to find the electrician had been before me to tesd them and had told the customer had to have the 2 replaced because they were "blown" and that he would now have to charge for 2 visits, one to test and...
  14. P

    immersion heating

    I've been asked to replace an immersion heating element , when I had a look at the hot water storage cylinder to get so starts of element it had non on it so I'm not to sure what length to get , is there any way you can tel what length you need without takingIit out, I would like to have...
  15. T

    Replacing an immersion heater thermostat with a cylinder thermostat.

    I am having a problem with the thermostat that is located in an immersion heater element fitted to a direct HW cylinder (located in an attic). It operates properly for a few weeks and then for some reason the stat trips. As the element is 27” long I guess that sometimes it can be prone to...
  16. M

    Immersion Heaters

    I have fitted 2 immersions to the same cylinder in the last 8 months. Both have blown. They are 11in and bottom immersions on a huge cylinder that takes 3 hours to drain down. My electrician friend has checked it and cannot give an electrical reason for this to happen. Has this happened to...
  17. M

    Hercules Direct Copper (IMI Range) Immersion heater went bang in the night

    Dear Forum Economy 7 immersion heater went bang in the night. It is an Hercules Dirrect Copper Cylinder IMI Range with two switches (it is about 20 years old) one at the bottom and one at the top. I have the bottom one switched on all the time as it stores during the night. I still have hot...
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  18. S

    No hot water and immersion heater too hot

    Hi all, right, dumbass here will try and relay the problem that I have. House is about 15 years old. Original Ideal Classic boiler in kitchen; honeywell stuff in airing cupboard (immersion, cylinder thermostat and 2- or 3-way motorised valve) and honeywell control in kitchen. I can get central...
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  19. B

    Immersion Heater programmer

    Hello, i have a customer who has asked me if i could supply them with a new Immersion heater programmer with a 5 minute boost facility. She used to be on economy 7 and has recently had solar pv and had the economy 7 taken off. She is very conscious about how much electric she will be using so...
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