If someone competent has gone to town on the likely causes of a problem I usually look at less likely possiblities that they may have dismissed prematurely.
E.g. you didn't mention the circulating pump or water pressure in the system. Is the pipework properly secured? Noise travels, particularly at 4am. Are you sure the boiler is responsible and not a dodgy TRV somewhere? Hot water cylinder coils can also make a number of strange singing / humming noises.
Do you have a recording of the noise?
E.g. you didn't mention the circulating pump or water pressure in the system. Is the pipework properly secured? Noise travels, particularly at 4am. Are you sure the boiler is responsible and not a dodgy TRV somewhere? Hot water cylinder coils can also make a number of strange singing / humming noises.
Do you have a recording of the noise?